Савремене методе масене спектрометрије у аналитици психоактивних супстанци у судској токсикологији
Лукић, Вера
Abstract
Mass spectrometry as an analytical technique has advanced extremely rapidly. New instruments with extraordinary characteristics have appeared on the market. Atmospheric ion sources have been developed, existing mass analyzers have been improved, and new hybrid instruments have been combined, the characteristics of which have raised mass spectrometry to an exceptional position by the analytical method. At the same time, newly designed psychoactive molecules are appearing on the drug market, which, together with new technologies, have influenced the work of toxicological laboratories. In the analysis of psychoactive substances, mass spectrometry is considered the method of choice. In this thesis modern instrumental techniques were used: GC-MS, GC-MS/MS, LC-MS/MS and LC-QTOF/MS in order to identify psychoactive substances in samples of importance in the field of forensic toxicology. Analytical parameters in tandem mass spectrometry were optimized; precursor ions, product ions, colision energy and reactions for monitoring analytes were selected, for the chosen psychoactive substances, so-called classic (morphine, codeine, 6-monoacetylmorphine) and newly designed drugs (from the group of synthetic cannabinoids, opioids, cathinone, phenethylamine, piperazine and аrylcyclohexylamines) in order to identify them. Criteria for the identification of psychoactive substances by mass spectrometry were also considered. The set methods for the identification of psychoactive substances were applied to real samples in forensic toxicology.
Abstract
Mass spectrometry as an analytical technique has advanced extremely rapidly. New instruments with extraordinary characteristics have appeared on the market. Atmospheric ion sources have been developed, existing mass analyzers have been improved, and new hybrid instruments have been combined, the characteristics of which have raised mass spectrometry to an exceptional position by the analytical method. At the same time, newly designed psychoactive molecules are appearing on the drug market, which, together with new technologies, have influenced the work of toxicological laboratories. In the analysis of psychoactive substances, mass spectrometry is considered the method of choice. In this thesis modern instrumental techniques were used: GC-MS, GC-MS/MS, LC-MS/MS and LC-QTOF/MS in order to identify psychoactive substances in samples of importance in the field of forensic toxicology. Analytical parameters in tandem mass spectrometry were optimized; precursor ions, product ions, colision energy and reactions for monitoring analytes were selected, for the chosen psychoactive substances, so-called classic (morphine, codeine, 6-monoacetylmorphine) and newly designed drugs (from the group of synthetic cannabinoids, opioids, cathinone, phenethylamine, piperazine and аrylcyclohexylamines) in order to identify them. Criteria for the identification of psychoactive substances by mass spectrometry were also considered. The set methods for the identification of psychoactive substances were applied to real samples in forensic toxicology.